2024-03-29T06:41:28Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2211452022-12-21T11:55:59Zcom_10261_68com_10261_2col_10261_321
Aznar-Moreno, José A.
Venegas-Calerón, Mónica
Du, Zhi-Yan
Garcés Mancheño, Rafael
Tanner, Julian A.
Chye, Mee-Len
Martínez-Force, Enrique
Salas, Joaquín J.
2020-10-14T10:00:12Z
2020-10-14T10:00:12Z
2020
Plant Science 300: 110630 (2020)
http://hdl.handle.net/10261/221145
10.1016/j.plantsci.2020.110630
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
Acyl-CoA-binding proteins (ACBP) bind to long-chain acyl-CoA esters and phospholipids, enhancing the activity of different acyltransferases in animals and plants. Nevertheless, the role of these proteins in the synthesis of triacylglycerols (TAGs) remains unclear. Here, we cloned a cDNA encoding HaACBP1, a Class II ACBP from sunflower (Helianthus annuus), one of the world's most important oilseed crop plants. Transcriptome analysis of this gene revealed strong expression in developing seeds from 16 to 30 days after flowering. The recombinant protein (rHaACBP1) was expressed in Escherichia coli and purified to be studied by in vitro isothermal titration calorimetry and for phospholipid binding. Its high affinity for saturated palmitoyl-CoA (16:0-CoA; K 0.11 μM) and stearoyl-CoA (18:0-CoA; K 0.13 μM) esters suggests that rHaACBP1 could act in acyl-CoA transfer pathways that involve saturated acyl derivatives. Furthermore, rHaACBP1 also binds to both oleoyl-CoA (18:1-CoA; K 6.4 μM) and linoleoyl-CoA (18:2-CoA; K 21.4 μM) esters, the main acyl-CoA substrates used to synthesise the TAGs that accumulate in sunflower seeds. Interestingly, rHaACBP1 also appears to bind to different species of phosphatidylcholines (dioleoyl-PC and dilinoleoyl-PC), glycerolipids that are also involved in TAG synthesis, and while it interacts with dioleoyl-PA, this is less prominent than its binding to the PC derivative. Expression of rHaACBP in yeast alters its fatty acid composition, as well as the composition and size of the host acyl-CoA pool. These results suggest that HaACBP1 may potentially fulfil a role in the transport and trafficking of acyl-CoAs during sunflower seed development.
openAccess
Acyl-CoA
Acyl-CoA-binding protein
Oilseeds
Phosphatidylcholine
Phospholipids
Sunflower
Triacylglycerol
Characterization and function of a sunflower (Helianthus annuus L.) Class II acyl-CoA-binding protein
artículo