2024-03-28T22:55:27Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1610212020-12-10T16:38:44Zcom_10261_56com_10261_3col_10261_309
Martín-Saldaña, Sergio
Palao-Suay, Raquel
Aguilar, María Rosa
García-Fernández, Luis
Arévalo, Humberto
Trinidad, Almudena
Ramírez-Camacho, Rafael
San Román, Julio
2018-02-20T12:23:49Z
2018-02-20T12:23:49Z
2018
Journal of controlled release : official journal of the Controlled Release Society 270: 53-64 (2018)
http://hdl.handle.net/10261/161021
10.1016/j.jconrel.2017.11.032
http://dx.doi.org/10.13039/501100010198
Polymeric nanoparticles (NPs) based on smart synthetic amphiphilic copolymers are used to transport and controlled release dexamethasone in the inner ear to protect against the ototoxic effect of cisplatin. The NPs were based on a mixture of two pseudo-block polymer drugs obtained by free radical polymerization: poly(VI-co-HEI) and poly(VP-co-MVE) or poly(VP-co-MTOS), being VI 1-vinylimidazole, VP N-vinylpyrrolidone, and HEI, MVE and MTOS the methacrylic derivatives of ibuprofen, α-tocopherol and α-tocopheryl succinate, respectively. The NPs were obtained by nanoprecipitation with appropriate hydrodynamic properties, and isoelectric points that matched the pH of inflamed tissue. The NPs were tested both in vitro (using HEI-OC1 cells) and in vivo (using a murine model) with good results. Although the concentration of dexamethasone administered in the NPs is around two orders of magnitude lower that the conventional treatment for intratympanic administration, the NPs protected from the cytotoxic effect of cisplatin when the combination of the appropriate properties in terms of size, zeta potential, encapsulation efficiency and isoelectric point were achieved. To the best of our knowledge this is the first time that pH sensitive NPs are used to protect from cisplatin-induced hearing loss by intratympanic administration.
eng
closedAccess
Antioxidant
Ototoxicity
Nanoparticles
Dexamethasone
Ibuprofen
Anti-inflammatory
pH-sensitive polymeric nanoparticles with antioxidant and anti-inflammatory properties against cisplatin-induced hearing loss
artículo