2024-03-29T07:07:42Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1441822019-06-03T07:57:04Zcom_10261_53com_10261_6col_10261_306
Mellado-Ortega, Elena
Zabalgogeazcoa, Iñigo
Vázquez de Aldana, Beatriz R.
Arellano, Juan B.
2017-02-17T11:12:41Z
2017-02-17T11:12:41Z
2016
Analytical Biochemistry (519): 27-29 (2017)
0003-2697
http://hdl.handle.net/10261/144182
10.1016/j.ab.2016.12.009
1096-0309
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100014180
Oxygen radical absorbance capacity (ORAC) assay in 96-well multi-detection plate readers is a rapid method to determine total antioxidant capacity (TAC) in biological samples. A disadvantage of this method is that the antioxidant inhibition reaction does not start in all of the 96 wells at the same time due to technical limitations when dispensing the free radical-generating azo initiator 2,2′-azobis(2-methyl propanimidamide) dihydrochloride (AAPH). The time delay between wells yields a systematic error that causes statistically significant differences in TAC determination of antioxidant solutions depending on their plate position. We propose two alternative solutions to avoid this AAPH-dependent error in ORAC assays.
spa
http://creativecommons.org/licenses/by-nc-nd/4.0/
openAccess
AAPH
ORAC
Peroxyl radical
Total antioxidant capacity
Trolox
96-well plate reader
Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay
artículo