2024-03-29T13:23:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1436482017-12-18T13:25:41Zcom_10261_108com_10261_8col_10261_361
Bezos, Javier
Álvarez, Julio
Juan, Lucía de
Romero, Beatriz
Rodriguez-Campos, Sabrina
Fernández de Mera, Isabel G.
Domínguez, Lucas
Aranaz, Alicia
2017-02-08T12:43:38Z
2017-02-08T12:43:38Z
2011
Preventive Veterinary Medicine 100(3-4): 187-192 (2011)
http://hdl.handle.net/10261/143648
10.1016/j.prevetmed.2011.03.012
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100006280
http://dx.doi.org/10.13039/501100014211
Caprine tuberculosis in Spain is mainly caused by Mycobacterium caprae although the progression of the disease and lesion severity is similar to that caused by Mycobacterium bovis. In this study, the sensitivity of the gamma-interferon (IFN-γ) assay using an antigen cocktail containing early secretory antigenic target-6. kDa (ESAT-6) and culture filtrate protein 10 (CFP-10) peptides for stimulation was determined and compared with those obtained in single intradermal tuberculin (SIT) and single intradermal cervical comparative tuberculin (SICCT) tests and IFN-γ assay using purified protein derivative (PPD) in three different flocks infected with M. caprae under different epidemiological conditions. Correlation between specific IFN-γ production and severity of lesions was also evaluated. Sensitivities of the diagnostic tests varied greatly in the three flocks studied, with higher values in those where higher lesion scores were observed. The results show that IFN-γ assay applied in goats using PPD or the ESAT-6/CFP-10 peptides cocktail for stimulation yielded similar sensitivity values. A significant yet weak positive correlation between specific IFN-γ production and lesion scores was detected after the stimulation with PPDs (p= 0.004) whereas when the blood samples were stimulated with ESAT-6/CFP-10 peptides, the correlation was not significant (p> 0.05). Therefore, specific-IFN-γ production after the stimulation with PPDs or ESAT-6/CFP-10 was not an accurate indicator of lesion severity in naturally tuberculosis infected goats with M. caprae.
eng
closedAccess
IFN-γ assay
Sensitivity
Tuberculosis
Lesion score
Diagnosis
Goat
Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks
artículo