2024-03-28T16:39:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1422982017-04-07T07:19:38Zcom_10261_118com_10261_6col_10261_1253
Carballo, Arkaitz
Murillo, Rosa
Williams, Trevor G.
Caballero, Primitivo
2017-01-10T07:49:00Z
2017-01-10T07:49:00Z
2016
IOBC-WPRS Bulletin 113: 127-130 (2016)
http://hdl.handle.net/10261/142298
Baculovirus are natural pathogens that can produce epizootics in agricultural ecosystems.
The Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control
this pest in sweet pepper crop systems of southern Spain. Studies on SeMNPV persistence
in covertly infected adults revealed the presence of RNA viruses in insect colonies. These
were identified as two iflaviruses (SeIV1 and SeIV2). Subsequent experiments indicated that
SeMNPV and SeIV could be detected in both field and laboratory insect populations using
PCR-based techniques. In this study, we evaluated the effect of SeIV1 co-infection on the
insecticidal characteristics of SeMNPV. For this, feeding-drop bioassays were performed on
second instar larvae by inoculating at the same time one of five SeMNPV inoculum concentrations and one of the following iflavirus treatments: i) SeIV1; ii) SeIV2; iii) SeIV1+SeIV2;
and mock-infected control. Overall, iflavirus co-inoculation consistently reduced median
lethal concentrations (LC50) for SeMNPV compared to larvae infected with SeMNPV alone.
However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. Adults survivors of a sublethal SeMNPV treatment were examined for covert infection and SeMNPV DNA were found to be present at a high prevalence in all treatments. In
conclusion, simultaneous SeMNPV-SeIV co-infection resulted in improved pathogenicity of
SeMNPV and did not alter the capacity of SeMNPV for vertical transmission.
eng
closedAccess
Effect of Spodoptera exigua iflavirus co-inoculation on the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
comunicaciĆ³n de congreso