2024-03-29T02:27:43Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1395662022-06-30T11:46:06Zcom_10261_79com_10261_1col_10261_332
Prado, Alicia del
Lázaro, José M.
Longás, Elisa
Villar, Laurentino
Vega, Miguel de
Salas, Margarita
2016-10-28T10:11:05Z
2016-10-28T10:11:05Z
2015
Journal of Biological Chemistry 290: 27138- 27145 (2015)
http://hdl.handle.net/10261/139566
10.1074/jbc.M115.682278
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/100008054
26400085
Background: The initiation of replication in φ29 mainly occurs opposite the 3' penultimate nucleotide of the template. Results: Mutations in the TP residue Phe-230 changed the specificity for the templating nucleotide. Conclusion: The results suggest a role for Phe-230 in determining the templating nucleotide. Significance: The results shed light on the positioning of the template nucleotide in organisms that initiate with proteinpriming mechanism.
eng
openAccess
Insights into the determination of the templating nucleotide at the initiation of φ29 DNA replication
artículo