2024-03-29T05:53:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1343832018-08-27T09:12:59Zcom_10261_63com_10261_6col_10261_442
González-Warleta, M.
Castro-Hermida, J. A.
Calvo, C.
Benavides, Julio
Regidor-Cerrillo, Javier
Ortega-Mora, Luis M.
Mezo, Mercedes
2016-07-04T11:11:24Z
2016-07-04T11:11:24Z
2015-06-30
ApiCOWplexa 2015 3rd lnternational Meeting on Apicomplexan Parasites in Farm Animals: 14 (2015)
http://hdl.handle.net/10261/134383
Neospora caninum has traditionally been considered an unimportant parasite in sheep.
However, recent studies carried out in Spain have shown that this protozoan can cause
severe reproductive failure in sheep. The importance of ovine neosporosis should
therefore be reconsidered and the mechan¡sms of transmission studied in depth. This
study aimed to evaluate the efficacy of endogenous transplacental transmission of N.
caninum in sheep. A total of 28 seroposit¡ve sheep were housed in facilities in which
horizontal infection was precluded. The sheep were mated, giving r¡se to 24 pregnant
sheep in which ultrasound scans (for pregnancy follow up) and blood sampl¡ng (for
antibody detection) were carried out monthly. Three of the sheep aborted 5 foetuses
and 21 of the sheep gave birth to 11 stillborn and 39 live lambs. Ten lambs were born
weak and died within a week of birth. Precolostral serum samples were collected from
all live newborn lambs for antibody detection. -Brain samples were obtained from all
aborted, stillborn and dead lambs for parasite DNA detection and histological analysis.
All sheep remained seropositive throughout pregnancy. Precolostral antibodies were
also detected in 38 out of 39 newborn lambs. Parasite DNA was detected in all dead
lambs (21) and in all foetuses except one (4/5). Scarce histological lesions consistent
with protozoan infection (glial foci) were observed in 16 brains. ln 13 out of these 16
cases, N. caninum tissue cysts were detected by immunohistochemistry. The results
show conclusively that endogenous transplacental transmission of neosporosis is highly
efficient in sheep.
eng
closedAccess
Endogenous transplacental trasnmission of Neospora caninum infection in naturally infected sheep
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