2024-03-28T22:42:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1286602022-03-24T13:25:25Zcom_10261_5063com_10261_5col_10261_5066
Haba, Ester
Pinazo Gassol, Aurora
Pons Pons, Ramon
Pérez, Lourdes
Manresa, Ángeles
2016-02-04T07:43:30Z
2016-02-04T07:43:30Z
2014-03-03
Biochimica et Biophysica Acta - Biomembranes
http://hdl.handle.net/10261/128660
10.1016/j.bbamem.2013.11.004
http://dx.doi.org/10.13039/501100003329
Rhamnolipids (RL) are one of the most important classes of biosurfactants produced by microorganisms using a wide range of carbon sources, from a simple carbon source like glucose to complex wastes such as the used cooking oils used in this work. The objective of this work was to learn about the rhamnolipid-phospholipid dipalmitoyl phosphatidyl choline (DPPC) molecular interactions through the behaviour observed in the neat products and four RL/DPPC mixtures. Size and z-potential were used to characterize the size and the charge of the vesicles, and small angle X-ray scattering (SAXS) was used to measure the vesicle bilayer characteristics, and the release of carboxyfluorescein to study the bilayer disrupting effect promoted by rhamnolipids. The results show that rhamnolipids are disposed in ordered bilayers with long repeating distances, which are stabilized by the charging of the bilayer and also by a strong fluidity of the bilayers. The ability of rhamnolipids to increase the fluidity of DPPC bilayers may be related with the strong haemolytic power of these molecules.
eng
http://www.sherpa.ac.uk/romeo/issn/0006-3002/
openAccess
Bilayer formation
Biosurfactants
DPPC
Physico-chemical property
Rhamnolipid
SAXS
Complex rhamnolipid mixture characterization and its influence on DPPC bilayer organization
artículo