2024-03-19T06:26:30Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1134632020-05-28T13:37:29Zcom_10261_38com_10261_5col_10261_291
Reyes-Darias, José Antonio
Sánchez-Luque, Francisco J.
Morales, Juan C.
Pérez-Rentero, Sonia
Eritja Casadellà, Ramón
Berzal-Herranz, Alfredo
2015-04-10T07:18:43Z
2015-04-10T07:18:43Z
2015
Chembiochem : a European journal of chemical biology 16: 584- 591 (2015)
http://hdl.handle.net/10261/113463
10.1002/cbic.201402574
© 2015 Wiley-VCH Verlag GmbH & Co. KGaA. Antisense oligodeoxynucleotides (ODNs) are short synthetic DNA polymers complementary to a target RNA sequence. They are commonly designed to halt a biological event, such as translation or splicing. ODNs are potentially useful therapeutic agents for the treatment of different human diseases. Carbohydrate-ODN conjugates have been reported to improve the cell-specific delivery of ODNs through receptor mediated endocytosis. We tested the anti-HIV activity and biochemical properties of the 5′-end glucose-conjugated GEM 91 ODN targeting the initiation codon of the gag gene of HIV-1 RNA in cell-based assays. The conjugation of a glucose residue significantly reduces the immunostimulatory effect without diminishing its potent anti-HIV-1 activity. No significant effects were observed in either ODN stability in serum, in vitro degradation of antisense DNA-RNA hybrids by RNase H, cell toxicity, cellular uptake and ability to interfere with genomic HIV-1 dimerisation.
eng
openAccess
Glucose DNA oligonucleotides
HIV-1 inhibition
Immune response reduction
Conjugated oligonucleotides
Glucose conjugation of anti-HIV-1 oligonucleotides containing unmethylated CpG motifs reduces their immunostimulatory activity
artículo