2024-03-28T12:33:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1091682022-12-15T08:51:29Zcom_10261_76com_10261_5col_10261_329
Cicarelli, R. M. B.
Khaouja, Amina
Codony, Carles
Espuny, Ruth
Eritja Casadellà, Ramón
Bach-Elias, Montse
2015-01-08T12:03:17Z
2015-01-08T12:03:17Z
1998-06-18
Biochemical and Biophysical Research Communications 247(2): 204-206 (1998)
http://hdl.handle.net/10261/109168
10.1006/bbrc.1998.8771
http://dx.doi.org/10.13039/100005156
We describe a defective HeLa nuclear extract which is particularly deficient in step 2 of splicing reaction. With this extract we have studied the conservation of a second-step activity from yeast to human cells. We detected a S. cerevisiae second-step splicing activity that allows restoration of step 2 of the defective HeLa nuclear extract, which indicates that yeast purified fraction has a second-step activity that is conserved from yeast to human cells. The activity is a yeast UsnRNP protein(s) since it is purified with anti-tri-methylguanosine by immunoaffinity columns.
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openAccess
A second-step splicing activity is conserved from yeast to human
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