2024-03-28T10:37:46Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1029952021-12-28T16:25:36Zcom_10261_64com_10261_1col_10261_317
Romero-López, Cristina
Barroso del Jesús, Alicia
García-Sacristán, Ana
Briones, Carlos
Berzal-Herranz, Alfredo
2014-10-07T11:16:18Z
2014-10-07T11:16:18Z
2013-09-17
Nucleic Acids Research
0305-1048
http://hdl.handle.net/10261/102995
10.1093/nar/gkt841
1362-4962
24049069
[EN]The hepatitis C virus (HCV) RNA genome contains multiple structurally conserved domains that make long-distance RNA–RNA contacts important in the establishment of viral infection. Microarray antisense oligonucelotide assays, improved dimethyl sulfate probing methods and 2′ acylation chemistry (selective 2’-hydroxyl acylation and primer extension, SHAPE) showed the folding of the genomic RNA 3′ end to be regulated by the internal ribosome entry site (IRES) element via direct RNA–RNA interactions. The essential cis-acting replicating element (CRE) and the 3′X-tail region adopted different 3D conformations in the presence and absence of the genomic RNA 5′ terminus. Further, the structural transition in the 3′X-tail from the replication-competent conformer (consisting of three stem-loops) to the dimerizable form (with two stem-loops), was found to depend on the presence of both the IRES and the CRE elements. Complex interplay between the IRES, the CRE and the 3′X-tail region would therefore appear to occur. The preservation of this RNA–RNA interacting network, and the maintenance of the proper balance between different contacts, may play a crucial role in the switch between different steps of the HCV cycle.
eng
http://www.oxfordjournals.org/en/oxford-open/charges.html
openAccess
End-to-end crosstalk within the hepatitis C virus genome mediates the conformational switch of the 3′X-tail region
artículo