2024-03-28T22:29:00Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/102672020-07-06T12:54:45Zcom_10261_5062com_10261_5col_10261_5064
Pérez Solsona, Sandra
McJury, Brenna E.
Eichhorn, Peter
Aga, Diana S.
2009-02-04T10:45:46Z
2009-02-04T10:45:46Z
2007-10-24
Journal of Chromatography A 1175(2): 234-241 (2007)
0021-9673
http://hdl.handle.net/10261/10267
10.1016/j.chroma.2007.10.053
This study aimed to develop and optimize a method for the extraction and analysis of moenomycin antibiotics (a.k.a. flavomycin) in corn-based feed premix and in chicken litter. Moenomycin-A was isolated from chicken litter using pressurized liquid extraction followed by a solid-phase extraction (SPE) clean-up step. The highly lipophilic nature of moenomycin necessitated the use of the less hydrophobic sorbent, C4-based SPE cartridge, and a higher temperature elution solvent, methanol at 50ºC, in order to obtain satisfactory percent recoveries. After clean-up, the sample was analyzed by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS). Various reversed-phase columns were examined, including C18, CN, perfluorinated C6, and a porous graphitic carbon. The set of conditions that gave the highest separation efficiency
while still maintaining symmetrical peak shape was the C18 column using the H2O + 0.3% HCOOH and acetonitrile mobile phase.
eng
closedAccess
Moenomycin
Liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS)
Chicken litter
Chicken feed
Determination of the antimicrobial growth promoter moenomycin-A in chicken litter
artículo