2024-03-29T10:28:19Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1012532018-09-19T10:04:51Zcom_10261_31891com_10261_2col_10261_31892
Ibáñez, Clara
Simó, Carolina
García-Cañas, Virginia
Gómez-Martínez, Ángeles
Ferragut, José A.
Cifuentes, Alejandro
2014-08-27T07:41:57Z
2014-08-27T07:41:57Z
2012
Electrophoresis 33(15): 2328-2336 (2012)
http://hdl.handle.net/10261/101253
10.1002/elps.201200143
http://dx.doi.org/10.13039/501100004837
In this study, an analytical multiplatform is presented to carry out a broad metabolomic study on the anti-proliferative effect of dietary polyphenols on human colon cancer cells. CE, RP/UPLC, and HILIC/UPLC all coupled to TOF MS were combined to achieve a global metabolomic examination of the effect of dietary polyphenols on HT29 colon cancer cells. By the use of a nontargeted metabolomic approach, metabolites showing significant different expression after the polyphenols treatment were identified in colon cancer cells. It was demonstrated that this multianalytical platform provided extensive metabolic information and coverage due to its complementary nature. Differences observed in metabolic profiles from CE-TOF MS, RP/UPLC-TOF MS, and HILIC/UPLC-TOF MS can be mainly assigned to their different separation mechanisms without discarding the influence of the different tools used for data processing. Changes in glutathione metabolism with an enhanced reduced glutathione/oxidized glutathione (GSH/GSSG) ratio were detected in polyphenols-treated cells. Moreover, significant alterations in polyamines content with important implications in cancer proliferation were observed after the treatment with polyphenols. These results from metabolomics can explain the chemopreventive effect of the tested dietary polyphenols on colon cancer and may be of importance for future prevention and/or treatment of this disease. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
eng
closedAccess
Metabolomics
CE-MS
UPLC-MS
Polyphenols
Colon cancer
CE/LC-MS multiplatform for broad metabolomic analysis of dietary polyphenols effect on colon cancer cells proliferation
artículo