2024-03-28T13:26:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/84242016-02-16T04:05:09Zcom_10261_79com_10261_1col_10261_836
DIGITAL.CSIC
advisor
Carrasco, Luis
author
Ramos, Marta
2008-11-11T14:59:12Z
2008-11-11T14:59:12Z
2007
http://hdl.handle.net/10261/8424
Fungi can cause a number of diseases ranking from localized mild infections to deep-seated
mycoses. Invasive fungal infections are more prevalent due to the increasing number of
high-risk patients, with different degrees of immunosuppression.
Nowadays, the diagnosis of fungal infections remains a significant problem. The clinical
presentation is difficult to interpret, and the findings of noninvasive methods (computed
tomographic scanning and X ray) are not specific. Culture results are available at the earliest
in 2 to 3 days, and blood and deep-tissue sample cultures from infections with focal lesions
are frequently negative. Direct microscopy and histopathological examination are rapid, but
they do not always allow identification of the infecting agent to the species level. In
contrast, even though the latest generation of monoclonal antibody-based enzyme-linked
immunosorbent assays (ELISAs) for circulating Aspergillus and Candida antigens are specific,
they lack sensitivity. Thus, rapid methods that are sensitive and specific are needed.
In this Doctoral Thesis a sensitive and specific PCR method and dot immunobinding assay
to detect very low levels of fungi components in blood samples have been successfully
developed.
On the other hand, despite efforts during the last few years to elucidate the cause of
AZOOR, multifocal choroiditis, serpiginous choroiditis and some neurodegenerative
diseases like multiple sclerosis, this has not been achieved. Intensive research for an
infectious agent that may directly provoke or trigger these diseases has been carried out in
many laboratories. A number of viruses, mostly from the herpesvirus group, have been
pointed out as the culprit. In the present PhD Thesis we have study the possibility that
AZOOR, multifocal choroiditis, serpiginous choroiditis and multiple sclerosis may have a
fungal origin.
Our results provide evidence that in most of the patients studied there are signs of fungal
infection. A high percentage of these patients contain fungal genomes and fungal antigens
in blood. Thus, some of these patients exhibit high antibody titers against several Candida
spp. and several of them contain significant amounts of β-1,3 glucan in serum.
Anyway, to distinguish if fungal infections are the cause or a consequence of these diseases,
it must be necessary to extend this study with a higher number of patients and to carry out
clinical trials with antifungal compounds.
spa
openAccess
Candidiasis
Técnicas de PCR
Diagnóstico de candidiasis mediante técnicas de PCR y de detección de antígenos fúngicos
tesis doctoral
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https://digital.csic.es/bitstream/10261/8424/1/Marta%20Ramos%20Rodrigo.pdf
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