2024-03-29T15:06:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/804382020-05-29T10:03:25Zcom_10261_123com_10261_8col_10261_502
DIGITAL.CSIC
author
Navarro-Martín, Laia
author
Viñas, Jordi
author
Ribas, Laia
author
Díaz, Noelia
author
Gutiérrez, Arantxa
author
Di Croce, Luciano
author
Piferrer, Francesc
2013-08-01T08:23:15Z
2013-08-01T08:23:15Z
2011-05-17
Comparative Morphology and Development Symposia (2010)
http://hdl.handle.net/10261/80438
Sex ratio shifts in response to temperature are common in fish and reptiles. However, the mechanism linking temperature during early development and sex ratios has remained elusive. We show in the European sea bass, a fish in which temperature effects on sex ratios are maximal before the gonads form, that males have double DNA methylation levels than females in the promoter of cyp19a, the gene coding for aromatase, the enzyme that converts androgens into estrogens. Exposure to high temperature increased the cyp19a promoter methylation levels of females, indicating that induced-masculinization involves DNA methylation-mediated control of aromatase gene expression, with an observed inverse relationship between methylation levels and expression. Although different CpGs within the cyp19a promoter exhibited different sensitivity to temperature, we show that the increased methylation of the cyp19a promoter, which occurs in the gonads but not in the brain, is not a generalized effect of temperature. Importantly, these effects were also observed in sexually undifferentiated fish, and were not altered by estrogen treatment. Thus, methylation of the cyp19a promoter is responsible for the lower expression of cyp19a in temperature-masculinized fish. In vitro, induced methylation of the cyp19a promoter suppressed the ability of SF-1 and Foxl2 to stimulate transcription. Finally, a CpG differentially methylated by temperature and adjacent to a Sox transcription factor binding site is conserved across species. Thus, DNA methylation of the aromatase promoter may be an essential component of the long-sought after mechanism connecting environmental temperature and sex ratios in species with temperature-dependent sex determination
eng
closedAccess
An epigenetic mechanism involved in temperature-induced sex ratio shifts in fish populations
póster de congreso
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