2024-03-28T12:21:26Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/578962018-08-16T11:53:27Zcom_10261_47com_10261_8col_10261_300
DIGITAL.CSIC
author
Tafalla, Carolina
author
Figueras Huerta, Antonio
author
Novoa, Beatriz
2012-10-11T10:52:58Z
2012-10-11T10:52:58Z
1999
Veterinary Immunology and Immunopathology 72(3-4): 249-256 (1999)
0165-2427
http://hdl.handle.net/10261/57896
10.1016/S0165-2427(99)00109-9
1873-2534
In the present work, we have studied the role of nitric oxide (NO) on the replication of viral
haemorrhagic septicemia virus (VHSV), a virus which produces high mortalities in fish aquaculture
worldwide and that is known to replicate in turbot (Scophthalmus maximus) head kidney
macrophages. Viral infection of turbot kidney macrophages in vitro induced an up-regulation of NO
production and we have tested whether this endogenous NO production induced by VHSV on
macrophages had an antiviral effect using the NO synthase inhibitor, N-!-nitro-L-arginine (LNAME).
When L-NAME was added to the VHSV-infected cultures, no increase on VHSV titer was
observed, eventhough the inhibitor was capable of decreasing NO production. When exogenous NO
was apported by the nitric oxide donor, glycerin trinitrate (GTN) an antiviral effect on VHSV was
observed. The NO donor significantly inhibited VHSV replication on a turbot fibroblast cell line
(TV-1) and on turbot kidney macrophages.
eng
closedAccess
Nitric oxide
Viral haemorrhagic septicemia virus
Macrophages
Turbot
Antiviral effect
Role of nitric oxide on the replication of viral haemorrhagic septicemia virus (VHSV), a fish rhabdovirus
artÃculo
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