2024-03-29T11:46:05Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/51692020-04-30T07:36:33Zcom_10261_35com_10261_5col_10261_288
DIGITAL.CSIC
author
Cebolla, Vicente L.
author
Mateos Serrano, Elena
author
Membrado, Luis
author
Vela, Jesús
author
Gálvez Buerba, Eva Mª
author
Matt, Muriel
author
Cossío, Fernando P.
2008-06-18T10:32:34Z
2008-06-18T10:32:34Z
2007-02-08
Journal of Chromatography - A 1146(2): 251-257 (2007)
0021-9673
http://hdl.handle.net/10261/5169
10.1016/j.chroma.2007.01.138
A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes.
eng
openAccess
Detection
Fluorescence scanning densitometry
Planar chromatography
Coralyne cation, a fluorescent probe for general detection in planar chromatography
artículo
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
URL
https://digital.csic.es/bitstream/10261/5169/1/J.%20Chromatogr.%20A%201146%20%282007%29.pdf
File
MD5
7b975cf8e3491dd617d55b0f9b6384a6
478226
application/pdf
J. Chromatogr. A 1146 (2007).pdf
URL
https://digital.csic.es/bitstream/10261/5169/4/J.%20Chromatogr.%20A%201146%20%282007%29.pdf.txt
File
MD5
b2a383a4e5bb727730df4c9a949422f4
28767
text/plain
J. Chromatogr. A 1146 (2007).pdf.txt