2024-03-28T23:13:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/473442018-09-12T11:14:03Zcom_10261_31891com_10261_2col_10261_31892
DIGITAL.CSIC
author
Miron, T. L.
author
Plaza, Merichel
author
Bahrim, G.
author
Ibáñez, Elena
author
Herrero, Miguel
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid
2012-03-22T11:31:01Z
2012-03-22T11:31:01Z
2011-07-29
Journal of Chromatography A 1218(30) : 4918-4927 (2011)
0021-9673
http://hdl.handle.net/10261/47344
10.1016/j.chroma.2010.11.055
1873-3778
http://dx.doi.org/10.13039/501100004837http://dx.doi.org/10.13039/100012818
In this contribution, pressurized liquid extraction (PLE) has been employed to isolate bioactive compounds from three native Romanian plants, oregano (Origanum vulgare), tarragon (Artemisia dracunculus) and wild thyme (Thymus serpyllum). Different PLE conditions have been tested including extraction with water, ethanol and their mixtures in a wide range of extraction temperatures (50–200 °C), and the antioxidant capacity of the extracts was measured using different assays (DPPH radical scavenging, TEAC assay and Folin–Ciocalteau assay to measure total phenols). Moreover, a complete chemical characterization by using LC–MS/MS was carried out to be able to correlate the bioactivity with the particular chemical composition of each extract and plant. The use of PLE with water as a solvent at the highest temperature tested (200 °C) always provided the highest extraction yields for the three studied plants, being maximum for oregano (>60%). Besides, oregano's pressurized water extracts at lower temperatures (50 °C) presented the highest content on total phenols (184.9 mg gallic acid/g extract) and the best antioxidant activities (EC50 6.98 μg/ml). In general, oregano extracts were the most active, followed by wild thyme extracts. The antioxidant capacity measured by DPPH assay was highly correlated with the amount of total phenols. Moreover, the use of a LC–MS/MS method allowed the identification of 30 different phenolic compounds in the different extracts, including phenolic acids, flavones, flavanones and flavonols, which have an important influence on the total antioxidant capacity of the different extracts.
eng
openAccess
Aromatic plants
Environmentally clean extraction techniques
LC–MS/MS
Phenolic antioxidants
Pressurized liquid extraction
Chemical composition of bioactive pressurized extracts of Romanian aromatic plants
artículo
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URL
https://digital.csic.es/bitstream/10261/47344/1/Chemical%20composition%20pressurized%20liquid%20extracts.pdf
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Chemical composition pressurized liquid extracts.pdf
URL
https://digital.csic.es/bitstream/10261/47344/4/Chemical%20composition%20pressurized%20liquid%20extracts.pdf.txt
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Chemical composition pressurized liquid extracts.pdf.txt