2024-03-29T12:54:10Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/250092020-01-15T09:26:30Zcom_10261_79com_10261_1col_10261_332
DIGITAL.CSIC
author
Bolívar Bolívar, Juan Manuel
author
Rocha-Martín, Javier
author
Mateo, César
author
Cava, Felipe
author
Berenguer, José
author
Fernández-Lafuente, Roberto
author
Guisán, José Manuel
funder
Comunidad de Madrid
funder
Ministerio de Educación y Ciencia (España)
funder
Fundación Ramón Areces
2010-06-04T18:20:19Z
2010-06-04T18:20:19Z
2009-04-13
Biomacromolecules 10(4): 742-7(2009)
0024-9297
http://hdl.handle.net/10261/25009
10.1021/bm801162e
http://dx.doi.org/10.13039/100008054http://dx.doi.org/10.13039/100012818
This paper shows a simple and effective way to avoid the dissociation of multimeric enzymes by coating their surface with a large cationic polymer (e.g., polyethylenimine (PEI)) by ionic exchange. As model enzymes, glutamate dehydrogenase (GDH) from Thermus thermophilus and formate dehydrogenase (FDH) from Pseudomonas sp. were used. Both enzymes are very unstable at acidic pH values due to the rapid dissociation of their subunits (half-life of diluted preparations is few minutes at pH 4 and 25 degrees C). GDH and FDH were incubated in the presence of PEI yielding an enzyme-PEI composite with full activity. To stabilize the enzyme-polymer composite, a treatment with glutaraldehyde was required. These enzyme-PEI composites can be crosslinked with glutaraldehyde by immobilizing previously the composite onto a weak cationic exchanger. The soluble GDH-PEI composite was much more stable than unmodified GDH at pH 4 and 30 degrees C (retaining over 90% activity after 24 h incubation) with no effect of the GDH concentration in the inactivation course. The composite could be very strongly, but reversibly, adsorbed on cationic exchangers. Similarly, FDH could be treated with PEI and glutaraldehyde after adsorption on cationic exchangers, This permitted a stabilized FDH preparation. In this way, the coating of the enzymes surfaces with PEI is used as a simple and efficient strategy to prevent enzyme dissociation of multimeric enzymes. These composites can be used as a soluble catalyst or reversibly immobilized onto a cationic exchanger (e.g., CM-agarose).
eng
closedAccess
Glutamate dehydrogenase (GDH)
Pseudomonas
Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes
artículo
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