2024-03-28T22:16:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/237922016-02-16T07:10:43Zcom_10261_22com_10261_1col_10261_275
DIGITAL.CSIC
author
Aragón, Juan J.
author
Sols, Alberto
2010-04-30T12:34:10Z
2010-04-30T12:34:10Z
1991
Faseb Journal 5(14): 2945-2950 (1991)
0892-6638
http://hdl.handle.net/10261/23792
The rapid development in our understanding of the regulation of enzyme activity makes it a high priority to ascertain whether the behavior of purified enzymes reflects their functional characteristics in vivo. Enzyme concentration is usually the most significant difference between routine in vitro assays and in vivo conditions, as it is well known that many intracellular enzymes are present in vivo at much higher concentrations than used in vitro. Various procedures are suitable for kinetic analysis at physiological concentrations of enzyme. Those more frequently used have been cell permeabilization, the utilization of purified enzymes at concentrations close to the in vivo range, and the addition of polyethylene glycol to increase the local protein concentration. In this review we briefly summarize observations on enzymes reported to exhibit concentration-dependent activity. The effect of enzyme concentration has been most thoroughly investigated in the case of phosphofructokinase. These studies may provide insight into the regulation of this important enzyme in the cell. The implications of both homologous and heterologous protein-protein interactions for the effect of enzyme concentration and their roles in the control of enzyme activity in vivo are also discussed.
eng
closedAccess
Enzyme regulation
Enzyme-enzyme interactions
Permeabilized cells
hosphofructokinase
Regulation of enzyme activity in the cell: effect of enzyme concentration
artículo
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URL
https://digital.csic.es/bitstream/10261/23792/3/accesoRestringido.pdf
File
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accesoRestringido.pdf
URL
https://digital.csic.es/bitstream/10261/23792/5/accesoRestringido.pdf.txt
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accesoRestringido.pdf.txt