2024-03-29T04:55:00Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2053712021-12-28T16:30:52Zcom_10261_74com_10261_6col_10261_327
DIGITAL.CSIC
author
Higuera-Sobrino, José Javier
author
Garrido Gala, José
author
Lekhbou, Ayman
author
Arjona Girona, Isabel
author
Amil-Ruiz, Francisco
author
Mercado Carmona, J. A.
author
Pliego-Alfaro, Fernando
author
Muñoz-Blanco, Juan
author
López Herrera, Carlos
author
Caballero Repullo, J. L.
funder
Junta de Andalucía
2020-03-26T16:53:23Z
2020-03-26T16:53:23Z
2019-04-18
Frontiers in Plant Science 10: 480 (2019)
http://hdl.handle.net/10261/205371
10.3389/fpls.2019.00480
1664-462X
http://dx.doi.org/10.13039/501100011011
31057583
Strawberry (Fragaria ×ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in strawberry to Colletotrichum acutatum. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, Agrobacterium-mediated transient transformation was used both to silence and overexpress the FaWRKY1 gene in strawberry fruits (Fragaria ×ananassa cv. Primoris), which were later analyzed upon C. acutatum inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for FaWRKY1 overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where FaWRKY1 was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when FaWRKY1 overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus C. acutatum in strawberry fruit and contrast with the previous role described for this gene in Arabidopsis as positive regulator of resistance against the bacteria Pseudomonas syringae. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon C. acutatum infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen.
eng
openAccess
WRKY transcription factor
Colletotrichum acutatum
Agrobacterium transformation
Strawberry fruit defense response
Fragaria ananassa
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection
artículo
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URL
https://digital.csic.es/bitstream/10261/205371/1/fpls-10-00480.pdf
File
MD5
0bfec29af8838ca9711f4cef3089a383
4320401
application/pdf
fpls-10-00480.pdf