2024-03-28T16:16:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1661192020-12-12T16:54:25Zcom_10261_134com_10261_1col_10261_1269
DIGITAL.CSIC
author
Muñoz-Barrera, Marta
author
Monje-Casas, Fernando
funder
Junta de Andalucía
funder
European Commission
funder
Ministerio de Economía y Competitividad (España)
2018-06-12T06:53:27Z
2018-06-12T06:53:27Z
2017
The Mitotic Exit Network: 35-44 (2017)
http://hdl.handle.net/10261/166119
10.1007/978-1-4939-6502-1_3
http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/501100011011
27826854
The budding index and the morphology of the spindle and the nucleus are excellent markers for the analysis of the progression through the different stages of the cell cycle in Saccharomyces cerevisiae. Here, we describe a protocol to evaluate the budding index in this model organism using phase contrast microscopy. We also describe an indirect immunofluorescence method designed for the visualization of microtubules and the nucleus in S. cerevisiae. Finally, we explain how both methodologies can be used in order to analyze cell cycle progression in the budding yeast.
eng
closedAccess
Nuclear morphology
Spindle
Budding index
Cell cycle
Mitotic exit
Microtubules
Immunofluorescence
Analysis of cell cycle progression in Saccharomyces cerevisiae using the budding index and tubulin staining
capítulo de libro
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URL
https://digital.csic.es/bitstream/10261/166119/1/accesoRestringido.pdf
File
MD5
42637ae8545636bc41605c1740a9a84e
15753
application/pdf
accesoRestringido.pdf