2024-03-28T12:19:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1635702020-12-10T17:16:35Zcom_10261_11773com_10261_1com_10261_128col_10261_11774col_10261_381
DIGITAL.CSIC
author
Pérez-Moreno, Juan J.
author
Espina-Zambrano, Agueda G.
author
García-Calderón, Clara B.
author
Estrada, Beatriz
funder
Junta de Andalucía
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Universidad Pablo de Olavide
2018-04-12T12:10:37Z
2018-04-12T12:10:37Z
2017
Journal of Cell Science 130(5): 950-962 (2017)
http://hdl.handle.net/10261/163570
10.1242/jcs.197459
http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/501100004837http://dx.doi.org/10.13039/501100011011
28104814
Cell-extracellular-matrix adhesion is mediated by cell receptors, mainly integrins and transmembrane proteoglycans, which can functionally interact. How these receptors are regulated and coordinated is largely unknown. We show that the conserved transmembrane Drosophila proteoglycan Kon-tiki (Kon, also known as Perdido) interacts with the αPS2βPS integrin (αPS2 is encoded by inflated and βPS by myospheroid) to mediate muscle-tendon adhesion. kon and inflated double mutant embryos show a synergistic increase in muscle detachment. Furthermore, Kon modulates αPS2βPS signaling at the muscle attachment, since phosphorylated Fak is reduced in kon mutants. This reduction in integrin signaling can be rescued by the expression of a truncated Kon protein containing its transmembrane and extracellular domains, suggesting that these domains are sufficient to mediate this signaling. We show that these domains are sufficient to properly localize the αPS2βPS ligand, Thrombospondin, to the muscle attachment, and to partially rescue Kon-dependent muscle-tendon adhesion.We propose that Kon can engage in a protein complex with αPS2βPS and enhance integrin-mediated signaling and adhesion by recruiting its ligand, which would increase integrin-binding affinity to the extracellular matrix, resulting in the consolidation of the myotendinous junction.
eng
openAccess
Extracellular matrix
Perdido
NG2
Myotendinous junction
Myogenesis
Muscle
Kon-tiki
Integrin
Chondroitin sulfate proteoglycans
CSPG4
Adhesion
Kon-tiki enhances PS2 integrin adhesion and localizes its ligand, Thrombospondin, in the myotendinous junction
artículo
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URL
https://digital.csic.es/bitstream/10261/163570/1/kontikiPS2.pdf
File
MD5
25b53fb41c5e65edbef755743bf03dc5
10525206
application/pdf
kontikiPS2.pdf