2024-03-28T11:04:18Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1537372020-10-20T12:11:54Zcom_10261_86com_10261_1col_10261_339
DIGITAL.CSIC
author
Thieme, T. G. M.
author
Buuk, C.
author
Gloyna, K.
author
Ortego, Félix
author
Farinós, Gema P.
funder
Monsanto Fund
2017-07-31T11:52:34Z
2017-07-31T11:52:34Z
2017-07-24
J Appl Entomol 42 (1-2)192-200(2017)
0931-2048
http://hdl.handle.net/10261/153737
10.1111/jen.12420
1439-0418
http://dx.doi.org/10.13039/100001247
From 2005 to 2015, Ostrinia nubilalis were collected in the most important maize-growing areas in Europe where MON 810 was cultivated. The susceptibility of these
O. nubilalis collections to the Cry1Ab protein was determined using overlay bioassays
and compared to that of reference (control) strains. Larvae that died or did not moult
after 7 days were used to calculate a moulting inhibition concentration (MIC). Two
different batches of Cry1Ab protein were used over the course of this study. Between
2005 and 2015, 145 collections of O. nubilalis from 14 areas were analysed. The
Cry1Ab susceptibility of populations from different geographic regions differed only
slightly across years. The greatest variability in the MIC50 for field samples collected
from 2005 to 2011 and tested with batch 1 was 6.6-fold in 2006. For field-collected
O. nubilalis, the difference between MIC50 values of the most susceptible and most
tolerant samples was 13.1-fold for this period. For samples collected in 2012–2015
and tested with batch 2, the greatest variability was 4.1-fold in 2014. A diagnostic
concentration (MIC99) was calculated for batch 1 (48 ng/cm2) using the results from all
the collections in 2005–2012. Bridging experiments indicated that the diagnostic concentration for batch 2 was 28 ng/cm2. From 2006 onwards, no O. nubilalis reached the 2nd larval stage when the diagnostic concentration of either batch of Cry1Ab was
used. Only one insect collected from Romania in 2012 and two insects collected as
reference strain from Spain in 2015 survived exposure to a dosage of 20 ng/cm2, and
none of these larvae survived on MON 810 maize. Our results indicate that there has
been no significant change in susceptibility to Cry1Ab in European populations of
O. nubilalis over the period 2005–2015.
eng
openAccess
Cry1Ab protein
Diagnostic concentration
European corn borer
Moulting inhibition concentration
Ten years of MON 810 resistance monitoring of field populations of Ostrinia nubilalis in Europe
artículo
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URL
https://digital.csic.es/bitstream/10261/153737/1/J.%20Appl.%20Entomology%202017.pdf
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J. Appl. Entomology 2017.pdf