2024-03-29T01:26:10Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1502012018-09-12T11:14:02Zcom_10261_31891com_10261_2col_10261_31892
DIGITAL.CSIC
author
Montero, Lidia
author
Sánchez-Camargo, A. P.
author
García-Cañas, Virginia
author
Cifuentes, Alejandro
author
Herrero, Miguel
author
Ibáñez, Elena
funder
Colciencias (Colombia)
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid
funder
European Commission
2017-05-23T12:25:45Z
2017-05-23T12:25:45Z
2016
Journal of Chromatography A 1428: 115-125 (2016)
http://hdl.handle.net/10261/150201
10.1016/j.chroma.2015.07.053
http://dx.doi.org/10.13039/501100004837http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/100012818
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12mg phloroglucinol equivalents g, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC×RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.
eng
closedAccess
Phenolic compounds
Sargassum muticum
Comprehensive LC
Phlorotannins
Brown algae
Anti-proliferative activity
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts
artículo
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URL
https://digital.csic.es/bitstream/10261/150201/1/accesoRestringido.pdf
File
MD5
42637ae8545636bc41605c1740a9a84e
15753
application/pdf
accesoRestringido.pdf