2024-03-29T00:15:01Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1486062021-12-28T15:49:11Zcom_10261_105com_10261_1col_10261_358
DIGITAL.CSIC
author
Fernández-Suárez, María E.
author
Escolà-Gil, Joan Carles
author
Pastor, Óscar
author
Dávalos, Antoni
author
Blanco-Vaca, Francisco
author
Lasunción, Miguel A.
author
Martínez-Botas, Javier
author
Gómez-Coronado, Diego
funder
Comunidad de Madrid
funder
European Cooperation in Science and Technology
funder
European Commission
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
2017-04-20T12:35:19Z
2017-04-20T12:35:19Z
2016-09-07
Scientific Reports 6: 32105 (2016)
http://hdl.handle.net/10261/148606
10.1038/srep32105
http://dx.doi.org/10.13039/501100000921http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100004587http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/100012818
27601313
Selective estrogen receptor modulators (SERMs) are widely prescribed drugs that alter cellular and whole-body cholesterol homeostasis. Here we evaluate the effect of SERMs on the macrophage-specific reverse cholesterol transport (M-RCT) pathway, which is mediated by HDL. Treatment of human and mouse macrophages with tamoxifen, raloxifene or toremifene induced the accumulation of cytoplasmic vesicles of acetyl-LDL-derived free cholesterol. The SERMs impaired cholesterol efflux to apolipoprotein A-I and HDL, and lowered ABCA1 and ABCG1 expression. These effects were not altered by the antiestrogen ICI 182,780 nor were they reproduced by 17β-estradiol. The treatment of mice with tamoxifen or raloxifene accelerated HDL-cholesteryl ester catabolism, thereby reducing HDL-cholesterol concentrations in serum. When [H]cholesterol-loaded macrophages were injected into mice intraperitoneally, tamoxifen, but not raloxifene, decreased the [H]cholesterol levels in serum, liver and feces. Both SERMs downregulated liver ABCG5 and ABCG8 protein expression, but tamoxifen reduced the capacity of HDL and plasma to promote macrophage cholesterol efflux to a greater extent than raloxifene. We conclude that SERMs interfere with intracellular cholesterol trafficking and efflux from macrophages. Tamoxifen, but not raloxifene, impair M-RCT in vivo. This effect is primarily attributable to the tamoxifen-mediated reduction of the capacity of HDL to promote cholesterol mobilization from macrophages.
eng
openAccess
Clinically used selective estrogen receptor modulators affect different steps of macrophage-specific reverse cholesterol transport
artículo
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URL
https://digital.csic.es/bitstream/10261/148606/1/Clinically%20used%20selective%20estrogen-Fernandez_Suarez.pdf
File
MD5
59239e93a9cec58286fec604fb96abc8
2774869
application/pdf
Clinically used selective estrogen-Fernandez_Suarez.pdf