2024-03-28T20:37:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1485272018-08-03T07:42:55Zcom_10261_74com_10261_6com_10261_59col_10261_453col_10261_438
DIGITAL.CSIC
author
Ortiz-Bustos, Carmen M.
author
Pérez-Bueno, María Luisa
author
Barón Ayala, Matilde
author
Molinero-Ruiz, Leire
2017-04-19T12:27:47Z
2017-04-19T12:27:47Z
2016-09
XVIII Congreso de la Sociedad Española de Fitopatología (2016)
http://hdl.handle.net/10261/148527
The holoparasitic plant Orobanche cumana Wallr. depends entirely on the sunflower to
obtain its supply of water, mineral nutrients and photoassimilates. The infection
becomes evident by emergence of broomrape stems around flowering time of sunflower.
However, the metabolism and water flow of the host plant are modified as a result of the
parasite attack earlier in the infection process. Imaging of blue-green fluorescence
(BGF) induced by UV light is a sensitive tool that provides information about changes
in the content of plant secondary metabolites as a consequence, among others, of biotic
stresses. On the other hand, infrared thermography can show alterations on leaf
temperature upon infection by plant pathogens. The objectives of this work were to
analyse the effect of the infection on sunflower plants by BGF and thermal imaging and
to evaluate the use of these techniques in the early detection of the infection, prior to the
broomrape emergence. Germinated seeds of sunflower susceptible to O. cumana were
inoculated by transplant to soil previously infested with parasite seeds. The controls
were sown in non-infested soil. Pots were grown in a glasshouse at 12–22°C for 5
weeks in a completely randomized design. Blue-green fluorescence (BGF) images were
acquired sequentially in developed leaf pairs along the initial four vegetative stages of
all the plants. Infrared and blue and green fluorescence (F440, F520, respectively, and
their ratio F440/F520) images of fully developed leaf pairs and unshaded leaves were
taken twice a week during three weeks. Moreover, pigment (chlorophylls and
carotenoids) contents of control and parasitized sunflower were determined
spectrophotometrically at the end of the experiment. Significant decreases in F440 and
F520 were observed in infected plants as early as two weeks after inoculation (wai), and
they remained significant along the whole experiment. Similarly, the value of
F440/F520 was lower than in the controls throughout the experiment. Concerning
pigments content, significant differences between inoculated and control plants were
obtained only for the fourth leaf pair. Finally, canopy temperature in infected plants was
consistently higher in all measurements. The decreases in fluorescence emission in
sunflower suggest a down-regulation of the secondary metabolism upon parasite
infection. On the other hand, increases in canopy temperature of infected plants point to
an alteration in sunflower transpiration due to stomatal closure as a response of
sunflower to the infection by O. cumana. To conclude, these results show that the nondestructive
techniques BGF and thermography could be used as indicators of broomrape
infection in sunflower plants as early as 2 wai.
eng
closedAccess
Blue and green fluorescence and thermal imaging in the early detection of sunflower broomrape (Orobanche cumana Wallr.)
comunicación de congreso
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https://digital.csic.es/bitstream/10261/148527/1/accesoRestringido.pdf
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accesoRestringido.pdf