2024-03-28T13:54:01Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1422272017-01-18T10:57:39Zcom_10261_118com_10261_6col_10261_497
DIGITAL.CSIC
author
Etxeberria, E.
author
González, Pedro
author
Pozueta Romero, Javier
2017-01-05T13:46:39Z
2017-01-05T13:46:39Z
2010-09
15th International Plant Membrane Biology Congress (2010)
http://hdl.handle.net/10261/142227
During transport of external solutes from
the apoplast to the vacuole by fluid-phase
endocytosis (FPE), endocytic vesicles me
rge with the tonoplast to release their
contents. The merging of endocytic vesicles with the vacuole generates in a larger
surface area/volume ratio, resulting in an
increase volume and dilution of vacuolar
contents. To concentrate solutes, a mechanism(s) to reduce tonoplast surface
area must exist. We investigated possible means of tonoplast rearrangement
during FPE using red beet hypocotyls
for their red and fluorescent vacuolar
pigment, which would identify any structure of vacuolar origin. When incubated
200 mM sucrose, cells took up sucrose in
part by endocytosis. Increase in 24 mM
sucrose content by endocytic sucrose uptake
implicated a 2% increase in vacuolar
volume and a 14% increase in tonoplast membrane surface area. During
endocytosis, we observed the formation
of vacuolar originating structures of
various sizes as demonstrated by confocal laser scanning microscopy. Therefore,
to counteract the disproportionate addition of membrane and fluids to the vacuole,
cells generate exocytic vesicles. Exocytic vesicles re-establish surface
area/volume ratio allowing for the concentration of solutes within the vacuole.
eng
closedAccess
Retrograde vesicles from the vacuole regulate tonoplast surface area and volume during fluid-phase endocitosis
póster de congreso
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URL
https://digital.csic.es/bitstream/10261/142227/1/accesoRestringido.pdf
File
MD5
42637ae8545636bc41605c1740a9a84e
15753
application/pdf
accesoRestringido.pdf