2024-03-29T00:42:03Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/131062017-07-04T12:50:23Zcom_10261_59com_10261_6col_10261_312
DIGITAL.CSIC
author
Terán, Wilson
author
Felipe, Antonia
author
Fillet, Sandy
author
Guazzaroni, María Eugenia
author
Krell, Tino
author
Ruiz, Raquel
author
Ramos, Juan L.
author
Gallegos, María Trinidad
2009-05-19T10:21:36Z
2009-05-19T10:21:36Z
2007-11-06
Molecular Microbiology (2007) 66(6), 1416–1428
0950-382X
http://hdl.handle.net/10261/13106
10.1111/j.1365-2958.2007.06004.x
Pseudomonas putida DOT-T1E, known for its high
tolerance to solvents, possesses three Resistance–
Nodulation–Cell Division-type (RND) efflux pumps,
namely TtgABC, TtgDEF and TtgGHI, which are
involved in the active extrusion of solvents. Expression
of the ttgABC and ttgGHI operons was previously
shown to be regulated by the adjacently
encoded repressors, TtgR and TtgV, respectively.
Upstream of the third RND operon, ttgDEF, is located
a putative regulator gene, ttgT. In this study, TtgT is
shown to bind to the promoter region of the ttgDEF
operon, and to be released from DNA in the presence
of organic solvents. In vitro studies revealed that TtgV
and TtgT bind the same operator sites in both the
ttgDEF and the ttgGHI promoters. However, the affinity
of TtgV for the ttgDEF operator was higher than
that of TtgT, which, together with the fact that the ttgV
promoter seems to be almost twice stronger than the
ttgT promoter, explains why TtgV takes over in the
regulation of the two efflux pump operons. The functional
replacement of the cognate, chromosomally
encoded TtgT by the plasmid-encoded paralogue
TtgV illustrates a new mode of efflux pump regulation
of which the physiological relevance is discussed.
eng
closedAccess
TtgV
TtgT
Pseudomonas putida
Complexity in efflux pump control: cross-regulation by the paralogues TtgV and TtgT
artículo
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