2024-03-28T15:02:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1297352020-12-10T07:45:16Zcom_10261_123com_10261_8col_10261_376
DIGITAL.CSIC
author
Galluzzi, Luca
author
Penna, Antonella
author
Bertozzini, Elena
author
Giacobbe, Maria Grazia
author
Vila, Magda
author
Garcés, Esther
author
Prioli, Silvia
author
Magnani, Mauro
funder
European Commission
funder
Ministero delle Politiche Agricole Alimentari e Forestali
2005-11
Harmful Algae 4(6): 973-983 (2005)
10261/129735
10.1016/j.hal.2005.01.004
http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100005401
Paralytic shellfish poisoning (PSP) is a syndrome caused by the consumption of shellfish contaminated with neurotoxins produced by organisms of the marine dinoflagellate genus Alexandrium. A. minutum is the most widespread species responsible for PSP in the Western Mediterranean basin. The standard monitoring of shellfish farms for the presence of harmful algae and related toxins usually requires the microscopic examination of phytoplankton populations, bioassays and toxin determination by HPLC. These procedures are time-consuming and require remarkable experience, thus limiting the number of specimens that can be analyzed by a single laboratory unit. Molecular biology techniques may be helpful in the detection of target microorganisms in field samples. In this study, we developed a qualitative PCR assay for the rapid detection of all potentially toxic species belonging to the Alexandrium genus and specifically A. minutum, in contaminated mussels. Alexandrium genus-specific primers were designed to target the 5.8S rDNA region, while an A. minutum species-specific primer was designed to bind in the ITS1 region. The assay was validated using several fixed seawater samples from the Mediterranean basin, which were analyzed using PCR along with standard microscopy procedures. The assay provided a rapid method for monitoring the presence of Alexandrium spp. in mussel tissues, as well as in seawater samples. The results showed that PCR is a valid, rapid alternative procedure for the detection of target phytoplankton species either in seawater or directly in mussels, where microalgae can accumulate. © 2005 Elsevier B.V. All rights reserved
eng
closedAccess
Alexandrium minutum
ITS
Mytilus galloprovincialis
PCR
rDNA
Development of a qualitative PCR method for the Alexandrium spp. (Dinophyceae) detection in contaminated mussels (Mytilus galloprovincialis)
artículo
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