2024-03-29T15:35:42Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1284972021-12-28T16:07:00Zcom_10261_79com_10261_1col_10261_332
DIGITAL.CSIC
author
Welnowska, Ewelina
author
Sanz, Miguel Ángel
author
Redondo, Natalia
author
Carrasco Llamas, Luis
funder
Fundación Ramón Areces
funder
Ministerio de Economía y Competitividad (España)
2016-02-02T09:22:56Z
2016-02-02T09:22:56Z
2011-07-14
PLoS ONE 6 (2011)
http://hdl.handle.net/10261/128497
10.1371/journal.pone.0022230
http://dx.doi.org/10.13039/100008054http://dx.doi.org/10.13039/501100003329
21779397
Previous work by several laboratories has established that translation of picornavirus RNA requires active eIF2α for translation in cell free systems or after transfection in culture cells. Strikingly, we have found that encephalomyocarditis virus protein synthesis at late infection times is resistant to inhibitors that induce the phosphorylation of eIF2α whereas translation of encephalomyocarditis virus early during infection is blocked upon inactivation of eIF2α by phosphorylation induced by arsenite. The presence of this compound during the first hour of infection leads to a delay in the appearance of late protein synthesis in encephalomyocarditis virus-infected cells. Depletion of eIF2α also provokes a delay in the kinetics of encephalomyocarditis virus protein synthesis, whereas at late times the levels of viral translation are similar in control or eIF2α-depleted HeLa cells. Immunofluorescence analysis reveals that eIF2α, contrary to eIF4GI, does not colocalize with ribosomes or with encephalomyocarditis virus 3D polymerase. Taken together, these findings support the novel idea that eIF2 is not involved in the translation of encephalomyocarditis virus RNA during late infection. Moreover, other picornaviruses such as foot-and-mouth disease virus, mengovirus and poliovirus do not require active eIF2α when maximal viral translation is taking place. Therefore, translation of picornavirus RNA may exhibit a dual mechanism as regards the participation of eIF2. This factor would be necessary to translate the input genomic RNA, but after viral RNA replication, the mechanism of viral RNA translation switches to one independent of eIF2. © 2011 Welnowska et al.
eng
openAccess
Translation of viral mRNA without active eIF2: The case of picornaviruses
artículo
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URL
https://digital.csic.es/bitstream/10261/128497/1/CarrascoL_TransllationOfViralmRNA.pdf
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