2024-03-28T23:30:40Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1243662021-12-27T16:32:01Zcom_10261_79com_10261_1col_10261_332
DIGITAL.CSIC
author
Martín, María José
author
Blanco Dávila, Luis
funder
CSIC-UAM - Centro de Biología Molecular Severo Ochoa (CBM)
funder
Fundación Ramón Areces
funder
Comunidad de Madrid
funder
Ministerio de Ciencia y Tecnología (España)
2015-11-03T11:55:13Z
2015-11-03T11:55:13Z
2014-05-30
Nucleic Acids Research 42: 7923- 7934 (2014)
http://hdl.handle.net/10261/124366
10.1093/nar/gku475
http://dx.doi.org/10.13039/100008054http://dx.doi.org/10.13039/501100006280http://dx.doi.org/10.13039/100012818
24878922
Human Polμ is a DNA polymerase belonging to the X family that has been implicated in the non-homologous end-joining (NHEJ) pathway during repair of double-strand breaks in DNA. Loop1 is a flexible piece of Polμ which has a critical role during terminal transferase and end-joining activities: it acts as a pseudo-template when the template strand is discontinuous or unavailable, whilst diffusing away if present to avoid steric clashes. Mutational analysis and inspection of the 3D structures available allowed us to identify a network of residues in charge of sensing the presence or absence of discontinuities in the template strand, which will in turn determine the final position adopted by Loop1. This network is formed by the previously uncharacterized thumb mini-loop (NSH motif) and the positively charged helix N, which contribute to the correct positioning of Loop1 and to juxtapose the discontinuous template strand during NHEJ of incompatible ends. Accordingly, single mutation of specific conserved residues in these motifs, whilst irrelevant in most of the cases for gap filling, largely affected terminal transferase and end-joining activities. Other point mutations in the 'hinges' of Loop1, such as residues Phe385 or Phe389, corroborated the flexibility requirements of this motif. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
eng
openAccess
Decision-making during NHEJ: A network of interactions in human Polμ implicated in substrate recognition and end-bridging
artículo
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https://digital.csic.es/bitstream/10261/124366/1/Blanco_Luis_Decision-makingDuringNHEJ.pdf
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