2024-03-28T23:17:18Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/999072018-09-19T12:11:43Zcom_10261_31891com_10261_2col_10261_31892
00925njm 22002777a 4500
dc
Gómez-Sala, B.
author
Recio, Isidra
author
Hernández, Pablo E.
author
2014
Cloning of the enterolysin A (EnlA) gene (enlA) from Enterococcus faecalis DAC9 into the pMSP3545-derived pMLG2-protein expression vector encoding EnlA under control of the inducible PnisA promoter permitted the controlled release and heterologous expression of mature EnlA by Lactococcus lactis subsp. cremoris NZ9000 and Lactococcus lactis subsp. lactis IL1403. The nisin-induced expression of enlA by L.lactis NZ9000 (pMLG2) and L.lactis IL1403 (pMLG2), grown in GM17 or bovine skim milk (BSM), caused a noticeable reduction of the optical density (OD600) of the cultures and death of the growing cells. However, a high angiotensin converting enzyme (ACE)-inhibitory activity (ACE-IA) was only observed in the BSM-derived hydrolysates of L.lactis IL1403 (pMLG2) after 48h-induction with nisin. Analysis of these hydrolysates by reversed phase high performance liquid chromatography-tandem mass spectrometry permitted the identification of major peptide fragments with known ACE-IA or sharing at least three C-terminal residues with those displaying ACE-IA. © 2013 Elsevier Ltd.
International Dairy Journal 34(1): 100-103 (2014)
http://hdl.handle.net/10261/99907
10.1016/j.idairyj.2013.07.011
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100002911
http://dx.doi.org/10.13039/100012818
Controlled enterolysin A-mediated lysis and production of angiotensin converting enzyme-inhibitory bovine skim milk hydrolysates by recombinant Lactococcus lactis