2024-03-29T08:58:22Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/734292016-11-18T09:20:39Zcom_10261_75com_10261_6col_10261_328
00925njm 22002777a 4500
dc
Peukert, Manuela
author
Matros, Andrea
author
Lattanzio, Giuseppe
author
Kaspar, Stephanie
author
Abadía Bayona, Javier
author
Mock, Hans-Peter
author
2012-02
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high-quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition.
Here, we present our current protocols for the detection of small molecules in cryodissected immature barley (Hordeum vulgare) grains and tobacco (Nicotiana tabacum) roots.
Examples of MALDI-MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described.
Finally, we discuss how MALDI-MSI can contribute to applied plant research.
Peukert M, Matros A, Lattanzio G, Kaspar S, Abadía J, Mock HP. Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). New Phytologist 193 (3): 806-815 (2012)
0028-646X
http://hdl.handle.net/10261/73429
10.1111/j.1469-8137.2011.03970.x
1469-8137
Barley grains
MALDI-MSI
sample preparation
small molecules
tobacco roots
Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)