2024-03-28T16:27:25Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/710042022-06-30T11:33:29Zcom_10261_54com_10261_1col_10261_307
00925njm 22002777a 4500
dc
Inan, M.
author
Blázquez-Llorca, Lidia
author
Merchán-Pérez, Ángel
author
Anderson, Stewart A.
author
DeFelipe, Javier
author
Yuste, Rafael
author
2013
Chandelier (or axo-axonic) cells are a distinct group of GABAergic interneurons that innervate the axon initial segments of pyramidal cells and thus could have an important role controlling the activity of cortical circuits. To understand their connectivity, we labeled upper layers chandelier cells (ChCs) from mouse neocortex with a genetic strategy and studied how their axons contact local populations of pyramidal neurons, using immunohistochemical detection of axon initial segments. We studied ChCs located in the border of layers 1 and 2 from primary somatosensory cortex and found that practically all ChC axon terminals contact axon initial segments, with an average of three to five boutons per cartridge. By measuring the number of putative GABAergic synapses in initial segments, we estimate that each pyramidal neuron is innervated, on average, by four ChCs. Additionally, each individual ChC contacts 35-50% of pyramidal neurons within the areas traversed by its axonal arbor, with pockets of very high innervation density. Finally, ChCs have similar innervation patterns at different postnatal ages (P18-P90), with only relatively small lateral expansions of their arbor and increases in the total number of their cartridges during the developmental period analyzed. We conclude that ChCs innervate neighboring pyramidal neurons in a dense and overlapping manner, a connectivity pattern that could enable ChCs to exert a widespread influence on their local circuits. © 2013 the authors.
Journal of Neuroscience 33: 1907- 1914 (2013)
http://hdl.handle.net/10261/71004
10.1523/JNEUROSCI.4049-12.2013
23365230
Dense and overlapping innervation of pyramidal neurons by chandelier cells