2024-03-29T09:36:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/492722021-12-28T15:54:21Zcom_10261_39com_10261_1col_10261_292
00925njm 22002777a 4500
dc
Astudillo, Alma M.
author
Pérez-Chacón, Gema
author
Meana, Clara
author
Balgoma, David
author
Pol, Albert
author
Pozo, Miguel A. del
author
Balboa, María A.
author
Balsinde, Jesús
author
2011-10
In this work we have studied the effect of caveolin-1 deficiency on the mechanisms that regulate free arachidonic acid (AA) availability. Results presented here demonstrate that macrophages from caveolin-1-deficient mice exhibit elevated fatty acid incorporation and remodeling and a constitutively increased CoA-independent transacylase activity. Mass spectrometry-based lipidomic analyses reveal stable alterations in the profile of AA distribution among phospholipids, manifested by reduced levels of AA in choline glycerophospholipids but elevated levels in ethanolamine glycerophospholipids and phosphatidylinositol. Furthermore, macrophages from caveolin-1 null mice show decreased AA mobilization and PGE 2 and LTB4 production upon cell stimulation. Collectively, these results provide insight into the role of caveolin-1 in AA homeostasis and suggest an important role for this protein in the eicosanoid biosynthetic response.
Journal of Biological Chemistry 286(40): 35299-35307 (2011)
0021-9258
http://hdl.handle.net/10261/49272
10.1074/jbc.M111.277137
1083-351X
21852231
Altered arachidonate distribution in macrophages from caveolin-1 null mice leading to a reduced eicosanoid synthesis