2024-03-28T11:39:17Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/48202016-02-16T03:09:46Zcom_10261_79com_10261_1col_10261_332
00925njm 22002777a 4500
dc
Domínguez-González, Irene
author
Vázquez-Cuesta, Silvia N.
author
Algaba, Alicia
author
Díez-Guerra, F. Javier
author
2007-02-12
Neurogranin (Ng) is a 78-amino-acid-long protein concentrated at dendritic spines of forebrain neurons that is involved in synaptic plasticity through the regulation of CaM (calmodulin)-mediated signalling. Ng features a central IQ motif that mediates binding to CaM and is phosphorylated by PKC (protein kinase C). We have analysed the subcellular distribution of Ng and found that it associates to cellular membranes in rat brain. In vitro binding assays revealed that Ng selectively binds to PA (phosphatidic acid) and that this interaction is prevented by CaM and PKC phosphorylation. Using the peptide Ng-(29–47) and a mutant with an internal deletion (Ng-IQless), we have shown that Ng binding to PA and to cellular membranes is mediated by its IQ motif. Ng expressed in NIH-3T3 cells accumulates at peripheral regions of the plasma membrane and localizes at intracellular vesicles that can be clearly visualized following saponin permeabilization. This distribution was affected by PLD (phospholipase D) and PIP5K (phosphatidylinositol 4-phosphate 5-kinase) overexpression. Based on these results, we propose that Ng binding to PA may be involved in Ng accumulation at dendritic spines and that Ng could modulate PA signalling in the postsynaptic environment.
Biochem. J. (2007) 404 (31–43)
0264-6021 (Print)
http://hdl.handle.net/10261/4820
http://dx.doi.org/10.13039/501100006280
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/100012818
Calmodulin
Neurogranin
Phosphatidic acid
Phosphatidylinositol 4,5-bisphosphate
Phospholipase D (PLD),
Protein kinase C (PKC)
Neurogranin binds to phosphatidic acid and associates to cellular membranes