2024-03-28T16:46:40Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/440372021-12-28T16:50:15Zcom_10261_9676com_10261_8col_10261_9677
00925njm 22002777a 4500
dc
Farias, Margaret E. M.
author
Jarvi, Susan I.
author
2010-05-27
Background
Avipoxvirus sp. is a significant threat to endemic bird populations on several groups of islands worldwide, including Hawaìi, the Galapagos Islands, and the Canary Islands. Accurate identification and genotyping of Avipoxvirus is critical to the study of this disease and how it interacts with other pathogens, but currently available methods rely on invasive sampling of pox-like lesions and may be especially harmful in smaller birds.
Methodology/Principal Findings
Here, we present a nested TaqMan Real-Time PCR for the detection of the Avipoxvirus 4b core protein gene in archived blood samples from Hawaiian birds. The method was successful in amplifying Avipoxvirus DNA from packed blood cells of one of seven Hawaiian honeycreepers with confirmed Avipoxvirus infections and 13 of 28 Hawaìi `amakihi (Hemignathus virens) with suspected Avipoxvirus infections based on the presence of pox-like lesions. Mixed genotype infections have not previously been documented in Hawaìi but were observed in two individuals in this study.
Conclusions/Significance
We anticipate that this method will be applicable to other closely related strains of Avipoxvirus and will become an important and useful tool in global studies of the epidemiology of Avipoxvirus.
PLoS ONE 5(5): e10745 (2010)
1932-6203
http://hdl.handle.net/10261/44037
10.1371/journal.pone.0010745
20523726
Taqman Real-Time PCR Detects Avipoxvirus DNA in Blood of Hawaìi `Amakihi (Hemignathus virens)