2024-03-29T01:30:00Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/379992018-10-17T08:28:31Zcom_10261_79com_10261_1col_10261_332
00925njm 22002777a 4500
dc
Salas, Margarita
author
Smith, Marvin A.
author
Stanley, Wendell M.
author
Wahba, Albert J.
author
Ochoa, Severo
author
1965-10-01
The assembly of polypeptide chains during protein biosynthe-
sis is believed to proceed from the NHz-terminal through the
COOH-terminal amino acid (l-4). Hence, the most direct
method for ascertaining the direction in which the genetic
message is read is to determine the end location o f a given
amino acid in polypeptide chains synthesized in a cell-free
system under the direction of synthetic polynucleotides having
a codon o f specified base sequence at one end o f the chain. Pre-
vious experiments (5) were inconclusive because o f (a) presence
o f nucleases in the system, (b) insufficient characterization o f the
polynucleotide messenger, and (c) difficulty o f performing end
group assays because of the insolubility of the phenylalanine
peptides formed. All of these obstacles have now been removed
through (a) the use o f a system low in nuclease activity con-
sisting o f purified Escherichia coli ribosomes and Lactobacillus
arabinosus supernatant and (b) the preparation and unequivocal
characterization o f short polyadenylic acid messengers with 1
cytidine residue (and therefore an AAC codon) at the 3’.end.
Lysine polypeptides are soluble in water and can be readily
characterized.
The Journal of Biological Chemistry 240: 3988-3995 (1965)
0021-9258
http://hdl.handle.net/10261/37999
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/100004352
Direction of reading of the genetic message