2024-03-28T18:53:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/30742013-03-05T13:35:21Zcom_10261_131com_10261_2col_10261_384
00925njm 22002777a 4500
dc
Puig, Sergi
author
Querol, Amparo
author
Barrio, Eladio
author
Pérez Ortín, José E.
author
2000-05
Natural strains of Saccharomyces cerevisiae are prototrophic homothallic yeasts that sporulate poorly, are often heterozygous, and may be aneuploid. This genomic constitution may confer selective advantages in some environments. Different mechanisms of recombination, such as meiosis or mitotic rearrangement of chromosomes, have been proposed for wine strains. We studied the stability of the URA3 locus of a URA3/ura3 wine yeast in consecutive grape must fermentations. ura3/ura3 homozygotes were detected at a rate of 1 x 10(-5) to 3 x 10(-5) per generation, and mitotic rearrangements for chromosomes VIII and XII appeared after 30 mitotic divisions. We used the karyotype as a meiotic marker and determined that sporulation was not involved in this process. Thus, we propose a hypothesis for the genome changes in wine yeasts during vinification. This putative mechanism involves mitotic recombination between homologous sequences and does not necessarily imply meiosis.
Applied and Environmental Microbiology 66 (5) : 2057-2061 (2000)
http://hdl.handle.net/10261/3074
Mitotic recombination
wine fermentation
vinification
Saccharomyces cerevisiae
Mitotic recombination and genetic changes in Saccharomyces cerevisiae during wine fermentation