2024-03-29T14:42:25Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2164592021-02-09T11:42:48Zcom_10261_59com_10261_6com_10261_53col_10261_312col_10261_306
00925njm 22002777a 4500
dc
Missbah El Idrissi, M.
author
Lamin, H.
author
ElFaik, S.
author
Tortosa Muñoz, Germán
author
Peix, Álvaro
author
Bedmar, Eulogio J.
author
Abdelmoumen, H.
author
2020
Aim: To analyse the diversity of nodule-forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results: Of the 31 bacterial strains, four were selected based on their REP-PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii-isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions: To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule-forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study: In this work, we show that Microvirga sp. can be isolated from root nodules of wild-grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.
Journal of Applied Microbiology 128: 1109- 1118 (2020)
http://hdl.handle.net/10261/216459
10.1111/jam.14526
http://dx.doi.org/10.13039/501100003476
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011011
Agriculture
Diversity
Microbial phylogenetics
Soil
Symbiosis
Microvirga sp. symbiovar mediterranense nodulates Lupinus cosentinii grown wild in Morocco