2024-03-28T19:08:44Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2158262021-03-05T05:32:54Zcom_10261_5063com_10261_5col_10261_5066
00925njm 22002777a 4500
dc
Leonardo, Sandra
author
Gaiani, Greta
author
Tsumuraya, Takeshi
author
Hirama, Masahiro
author
Turquet, Jean
author
Sagristà, Núria
author
Rambla-Alegre, Maria
author
Flores, Cintia
author
Caixach, Josep
author
Diogène, Jorge
author
O'Sullivan, Ciara K.
author
Alcaraz, Carles
author
Campàs, Mònica
author
2020-03-05
The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PolyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 51-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 μg/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.
Analytical Chemistry 92 (7): 4858–4865 (2020)
http://hdl.handle.net/10261/215826
10.1021/acs.analchem.9b04499
http://dx.doi.org/10.13039/501100003329
Immunology
Sensors
Addressing the analytical challenges for the detection of ciguatoxins using an electrochemical biosensor