2024-03-28T16:49:18Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2156832021-12-28T15:35:24Zcom_10261_31891com_10261_2col_10261_31892
00925njm 22002777a 4500
dc
Muñoz-Almagro, Nerea
author
Gilbert-López, Bienvenida
author
Pozuelo Rollón, María del Carmen
author
García-Fernandez, Yolanda
author
Almeida, Carlos
author
Villamiel, Mar
author
Mendiola, J. A.
author
Ibáñez, Elena
author
2020-06-12
In the present study, the chemical composition of the microalga Euglena cantabrica was investigated. The extraction of bioactive compounds was done using pressurized liquid extraction (PLE) at different temperatures (40–180 °C) and using green solvents (ethanol-water mixtures). A statistical design of experiments was used to optimize the maximum antioxidant capacity of the extracts by response surface methodology. The antioxidant capacity was determined through the inhibition of 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, while the chemical analyses of the extracts were carried out using different chromatographic techniques. Chlorophylls and carotenoids were analyzed by high-performance liquid chromatography coupled to a diode array detector and mass spectrometry (HPLC-DAD-MS/MS) and carbohydrates by gas chromatography with flame ionization detection (GC-FID) and high-pressure size-exclusion chromatography coupled to an evaporative light-scattering detector (HPSEC-ELSD). The results showed different possibilities for the extraction conditions, depending on the desired bioactivity or chemical composition. Briefly, (i) mixtures of ethanol-water containing around 40% ethanol at 180 °C gave the best antioxidant capacity, (ii) mixtures containing around 50% ethanol at 110 °C gave the best yield of β-glucan paramylon, and (iii) the use of pure ethanol at a low temperature (40 °C) is the best choice for the recovery of carotenoids such as diatoxanthin. Summing up, E. cantabrica seems to be a good candidate to be used in biorefinery to obtain different bioactive compounds
Marine Drugs 18(6): 308 (2020)
1660-3397
http://hdl.handle.net/10261/215683
10.3390/md18060308
1660-3397
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000780
32545497
Euglena cantabrica
Pressurized liquid extraction
Carotenoids
Carbohydrates
Response surface
Microalga
Paramylon
Exploring the microalga Euglena cantabrica by pressurized liquid extraction to obtain bioactive compounds