2024-03-29T14:07:46Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/207052021-05-11T00:46:17Zcom_10261_74com_10261_6col_10261_327
00925njm 22002777a 4500
dc
Verberne, Marianne C.
author
Verpoorte, Rob
author
Bol, John F.
author
Mercado-Blanco, Jesús
author
Linthorst, Huub J. M.
author
2000-07
After a hypersensitive response to invading pathogens, plants show elevated accumulation of salicylic
acid (SA), induced expression of plant defense genes, and systemic acquired resistance (SAR) to further
infection by a broad range of pathogens. There is compelling evidence that SA plays a crucial role in triggering
SAR. We have transformed tobacco with two bacterial genes coding for enzymes that convert
chorismate into SA by a two-step process. When the two enzymes were targeted to the chloroplasts, the
transgenic (CSA, constitutive SA biosynthesis) plants showed a 500- to 1,000-fold increased accumulation
of SA and SA glucoside compared to control plants. Defense genes, particularly those encoding
acidic pathogenesis-related (PR) proteins, were constitutively expressed in CSA plants. This expression
did not affect the plant phenotype, but the CSA plants showed a resistance to viral and fungal infection resembling SAR in nontransgenic plants.
Nature Biotechnology 18, 779-783 (2000)
1087-0156
http://hdl.handle.net/10261/20705
10.1038/77347
Salicylic acid
Systemic acquired resistance
Tobacco
Tobacco mosaic virus
Oidium lycopersicon
PR gene expression
Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance