2024-03-28T18:58:39Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/203292019-02-28T08:04:12Zcom_10261_5063com_10261_5col_10261_5066
00925njm 22002777a 4500
dc
Segura, Mireia
author
Ortuño, Jordi
author
Farré-Albaladejo, Magi
author
Pacifici, Roberta
author
Pichini, Simona
author
Joglar Tamargo, Jesús
author
Segura, Jordi
author
Torre, Rafael de la
author
2003-05-22
A high-performance liquid chromatography (HPLC) method with tandem mass spectrometric detection is described for the determination of paroxetine, an antidepressant drug, and its metabolite (3S,4R)-4-(4-fluorophenyl)-3-(4-hydroxy-3-methoxyphenoxymethyl)piperidine (HM paroxetine) in human plasma. Plasma samples were hydrolysed with hydrochloric acid and then analytes were extracted with ethyl acetate at alkaline pH. Extracts were analysed by HPLC coupled to an atmospheric pressure ionisation-electrospray (ESI) interface and an ion trap mass spectrometer. Chromatography was performed on a reversed-phase column using acetonitrile/0.02% formic acid (66:34, v/v) as a mobile phase. The mass spectrometer was operated in the multiple reaction monitoring mode. The method was validated over concentration ranges of 0.75-100 g/L and 5-100 g/L for paroxetine and HM paroxetine, respectively. Mean recoveries of 77% for paroxetine and 76% for HM paroxetine were found, with precision always better than 15%. The limits of detection and quantification were 0.20 and 0.70 g/L for paroxetine, and 0.70 and 2.20 g/L for its metabolite. The method was applied to the analysis of plasma samples obtained from nine healthy male volunteers administered with a single oral dose of 20 mg paroxetine. After the 20-mg dose, the mean peak plasma concentration was 8.60 g/L for paroxetine and 92.40 g/L for HM paroxetine showing a tenfold ratio between the metabolite and the parent drug along the entire time-concentration curve.
Rapid Communications in Mass Spectrometry 17(13): 1455-1461 (2003)
0951-4198
http://hdl.handle.net/10261/20329
10.1002/rcm.1067
1097-0231
High-Performance Liquid chromatography (HPLC)
Paroxetine
Pharmacokinetic properties
Plasma concentrations
Quantitative determination of paroxetine and its 4-hydroxy-3-methoxy metabolite in plasma by high-performance liquid chromatography/electrospray ion trap mass spectrometry: application to pharmacokinetic studies