2024-03-28T12:33:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1638252020-07-08T12:04:20Zcom_10261_101com_10261_5col_10261_354
00925njm 22002777a 4500
dc
Pita, Marcos
author
Gutiérrez Sánchez, Cristina
author
Natale, Paolo
author
García-Molina, Gabriel
author
Márquez, Ileana F.
author
Marques, Marta C.
author
Zacarias, Sonia
author
Pereira, Inês A. C.
author
López-Montero, Iván
author
Vélez, Marisela
author
López de Lacey, Antonio
author
2017-11-29
Cells generate energy by coupling a proton gradient across a phospholipid bilayer membrane
with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an
artificial environment, we show that ATP can be efficiently produced starting from molecular
hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be
controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]-
hydrogenase from Desulfovibrio vulgaris Hildenborough [1]. The electro-enzymatic oxidation of H2
generated a proton gradient across the supported biomimetic membrane that can be coupled to the
in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system [2].
Such a system is also suitable for developing an electrochemical biosensor of ATP.
Proceedings 1(8): 766 (2017)
2504-3900)
http://hdl.handle.net/10261/163825
10.3390/proceedings1080766
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001871
http://dx.doi.org/10.13039/501100000781
http://dx.doi.org/10.13039/501100003339
ATP Synthesis and Biosensing Coupled to the Electroenzymatic Activity of a Hydrogenase on an Electrode/Biomimetic Membrane Interface