2024-03-29T13:32:29Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1438432022-01-18T08:48:08Zcom_10261_47com_10261_8com_10261_125com_10261_2col_10261_426col_10261_504
00925njm 22002777a 4500
dc
Cal, Laura
author
Gómez-Marín, Carlos
author
Gómez-Skarmeta, José Luis
author
Cerdá-Reverter, José Miguel
author
Kelsh, Robert N.
author
Rotllant, Josep
author
2015-06-25
Pigment abnormalities are one of the leading causes in performance loss in at sh aquaculture.
Nevertheless, and in spite of the importance of the problem, there is scanty information
about the molecular regulation of sh pigmentation which makes the control of this problem
di cult. To date, the most important regulator of skin and hair pigmentation in vertebrates is
the Agouti signaling protein (ASIP)- melanocortin 1 receptor (MC1R) system: the agouti locus
encodes the Agouti-signaling protein (ASIP), which antagonizes the e ects of -Melanocyte-
Stimulating hormone (-MSH) binding to the MC1R on the follicular melanocyte, resulting
in a decrease in the production of eumelanins (dark pigments) and an increase in pheomelanins
(red/yellow pigments). A key aspect of mammalian pigment pattern, that is widely
conserved, is the pale ventrum and darker dorsum; the underlying mechanism is regulation
of ASIP expression, with high ventral Agouti driving pale belly color. Likewise, sh often show
a pale ventrum, but here the pigmentation mechanism is very di erent with independent
cell-types, each expressing chemically distinct pigments (not just melanins), being di erentially
distributed. Recently, we have shown that not only the agouti gene (asip1), but also
even the elevated ventral expression levels, are conserved in sh. It is of interest, in order to
know the molecular regulation of sh pigmentation, to establish how Asip1 functions to regulate
sh pigment pattern formation. In order to explore the mechanism of Asip1 in zebra sh
pigment pat tern formation, we used a BAC transgenic approach to allow characterization of
asip1 cis-regulation regions. We have generated a BAC transgenic zebra sh line in which 5
and 3 agouti locus regulatory regions direct eGFP overexpression. Our initial observations
showed a graded dorso-ventral expression of eGFP. Therefore, eGFP expression in the asip1-
iTol2-eGFP-BAC transgenic sh faithfully recapitulated the endogenous expression pattern
of asip1 mRNA. This opens the way to characterizing the mechanisms generating this crucial
graded distribution of asip1
The International Symposium on Genetics in Aquaculture XII (2015)
http://hdl.handle.net/10261/143843
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100003339
Dorso-ventral gradient
Transgenesis
Zebra sh
Agouti
Pigmentation
A Bac transgenic analysis of the asip1 locus reveals developmental mechanisms of dorso-ventral pigmentation in fish.