2024-03-28T17:56:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1381262016-12-22T09:19:02Zcom_10261_70com_10261_2col_10261_323
00925njm 22002777a 4500
dc
Navarro, Marta
author
Morales, F. J.
author
2016-02-08
Advanced glycation end products (AGEs) are involved in the aging and the development of common chronic diseases. Hydroxytyrosol (HT) and its acetate derivative (HTA) exert a significant inhibitory activity on the formation of fluorescent AGEs in bovine serum albumin glycation model systems induced by methylglyoxal (IC50 value of 0.48 and 0.58 µmol/mL, respectively) and glucose (IC50 2.30 and 2.92 µmol/mL, respectively). Furthermore, HT and HTA showed a relevant carbonyl scavenging capacity toward methylglyoxal and glyoxal, which are the most potent promoters of the glycation in vivo, at molar reaction rates from 0.2 to 10 (carbonyl:phenol). However, carbonyl trapping capacity was significantly more effective against methylglyoxal (IC50 0.19 µmol/mL) than glyoxal (IC50 0.26 µmol/mL). At equimolar concentrations, the ester linkage did not significantly affect the antiglycative activity and carbonyl trapping capacity of the orthodiphenolic ring structure. Results were confirmed with the specific inhibition of the formation of the principal AGEs. Formation of carboxymethyl-lysine, argpyrimidine and carboxyethyl-lysine was significantly reduced by 61.9, 71.4 and 20.9 %, respectively. HT and its esters could be considered for upscaling studies as promising natural strategy against adverse consequences of protein glycation.
European Food Research and Technology A, 242,7:1101-110 (2016)
1438-2377
http://hdl.handle.net/10261/138126
10.1007/s00217-015-2614-8
1438-2385
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/100012818
Hydroxytyrosol
Antiglycative activity
Advanced glycation end products
Carboxymethyl-lysine
Carboxyethyl-lysine
Argpyrimidine
In vitro investigation on the antiglycative and carbonyl trapping activities of hydroxytyrosol