2024-03-28T20:53:01Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1290862021-12-27T15:39:22Zcom_10261_59com_10261_6com_10261_33com_10261_5com_10261_31891com_10261_2col_10261_312col_10261_286col_10261_31892
00925njm 22002777a 4500
dc
Martín-Ortiz, Andrea
author
Salcedo, J.
author
Barile, Daniela
author
Bunyatratchata, A.
author
Moreno, F. Javier
author
Martín-García, I.
author
Clemente, Alfonso
author
Sanz, M. Luz
author
Ruiz-Matute, Ana I.
author
2016
A detailed qualitative and quantitative characterization of goat colostrum oligosaccharides (GCO) has been carried out for the first time. Defatted and deproteinized colostrum samples, previously treated by size exclusion chromatography (SEC) to remove lactose, were analyzed by nanoflow liquid chromatography–quadrupole-time of flight mass spectrometry (Nano-LC-Chip–Q-TOF MS). Up to 78 oligosaccharides containing hexose, hexosamine, fucose, N-acetylneuraminic acid or Nglycolylneuraminic acid monomeric units were identified in the samples, some of them detected for the first time in goat colostra. As a second step, a hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) methodology was developed for the separation and quantitation of the main GCO, both acidic and neutral carbohydrates. Among other experimental chromatographic conditions, mobile phase additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry of target carbohydrates. Narrow peaks (wh: 0.2–0.6 min) and good symmetry (As: 0.8–1.4) were obtained for GCO using an acetonitrile:water gradient with 0.1% ammonium hydroxide at 40 ◦C. These conditions were selected to quantify the main oligosaccharides in goat colostrum samples. Values ranging from 140 to 315 mg L−1 for neutral oligosaccharides and from 83 to 251 mg L−1 for acidic oligosaccharides were found. The combination of both techniques resulted to be useful to achieve a comprehensive characterization of GCO.
Journal of Chromatography A 1428: 143-153 (2016)
0021-9673
http://hdl.handle.net/10261/129086
10.1016/j.chroma.2015.09.060
1873-3778
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100011011
26427327
Goat colostrum
Oligosaccharides
Mass spectrometry
Hydrophilic interaction liquid chromatography
Characterization of goat colostrum oligosaccharides by nano-liquid chromatography on chip quadrupole time-of-flight mass spectrometry and hydrophilic interaction liquid chromatography-quadrupole mass spectrometry