2024-03-19T10:17:05Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1290792021-12-28T16:08:13Zcom_10261_128com_10261_1col_10261_381
00925njm 22002777a 4500
dc
Irimia, Manuel
author
Royo, José Luis
author
Burguera, Demian
author
Maeso, Ignacio
author
Gómez-Skarmeta, José Luis
author
Garcia-Fernàndez, Jordi
author
2012
The origin and evolution of the complex regulatory landscapes of some vertebrate developmental genes, often spanning hundreds of Kbp and including neighboring genes, remain poorly understood. The Sonic Hedgehog (Shh) genomic regulatory block (GRB) is one of the best functionally characterized examples, with several discrete enhancers reported within its introns, vast upstream gene-free region and neighboring genes (Lmbr1 and Rnf32). To investigate the origin and evolution of this GRB, we sequenced and characterized the Hedgehog (Hh) loci from three invertebrate chordate amphioxus species, which share several early expression domains with Shh. Using phylogenetic footprinting within and between chordate lineages, and reporter assays in zebrafish probing >30 Kbp of amphioxus Hh, we report large sequence and functional divergence between both groups. In addition, we show that the linkage of Shh to Lmbr1 and Rnf32, necessary for the unique gnatostomate-specific Shh limb expression, is a vertebrate novelty occurred between the two whole-genome duplications.
Scientific Reports 2: 433 (2012)
http://hdl.handle.net/10261/129079
10.1038/srep00433
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100003741
http://dx.doi.org/10.13039/501100011011
22666536
Comparative genomics of the Hedgehog loci in chordates and the origins of Shh regulatory novelties